How To
Submit Request
To make reservations for assisted flow cytometry acquisition, please make reservations at least 48 hours in advance. Contact us for scheduling.
For any other service, a filled order request form is required before any service is performed. Projects with less than 20 samples can usually be completed within 2 weeks of order placement and sample delivery. For projects with more samples, please contact us for turnaround time.
- Download and complete the appropriate service request form electronically:
- For Luminex, Simoa, ELISA and xCELLigence assays: Immunoassay and xCELLigence order form
- For flow cytometry-based assays and sample processing: Flow cytometry and sample processing form
- Email the completed form as an attachment to [email protected]. We will follow up by email regarding your request.
Sample Preparation
Click on the links below for assay-specific instructions:
- Flow cytometry-based immune assessment
- Immunoassays (Luminex, Simoa and ELISA)
- Real-Time Cell Lysis and Proliferation using xCELLigence
Please visit our Services and Pricing page for specific instructions regarding collecting samples and planning experiments. For further consultation for experimental design, please contact us .
Sample Submission
On-Campus Sample Drop-Off
Prior to drop-off, please contact the laboratory at (310) 794-8932 to schedule delivery.
1000 Veteran Ave., Rehab Center 1-308
Los Angeles, CA 90095-1732
Mail Code: 168017
(310) 206-0270
Monday–Friday, 8:00 AM–6:00 PM
Off-Campus Sample Shipping
Prior to shipping, please contact the laboratory at (310) 794-8932 to schedule delivery. At the time of shipping, please email [email protected] with the tracking number.
It is preferred that samples be sent overnight before Wednesday to guarantee receipt by Friday. Clients will be notified by email when samples are received.
UCLA Immune Assessment Core
1000 Veteran Ave., Rehab Bldg. 1-308
Los Angeles, CA 90095-1735
Results and Data Retrieval
Click on the links below for assay-specific instructions:
- Flow cytometry-based immune assessment
- Imunoassays (Luminex and ELISA)
- Real-Time Cell Lysis and Proliferation using xCELLigence
For further consultation regarding results and data analysis, please contact us.
Flow cytometry-based immune assessment
Sample Preparation
All basic requirements for each available assay can be found in the Table below. Our assays are optimized for use with fresh blood or isolated human PBMCs/mouse splenocytes. Please inquire about requests for experiments using other sources of cells.
| Assay | Acceptable Specimen | Acceptable Time from Collection (for whole blood only) | Minimum Test Requirements |
|---|---|---|---|
| Immunophenotyping | Peripheral whole blood collected in ACD, sodium/lithium heparin, or EDTA | 30 hrs | 200 µL blood or 0.5–1 x 106 PBMCs (per panel) |
| Intracellular Cytokine Staining | Peripheral whole blood collected in ACD or sodium/lithium heparin | 30 hrs | 2 mL blood or 2 x 106 PBMCs (per stimulus) |
| Direct and Indirect All-stimulation Assay | Peripheral whole blood collected in ACD or sodium/lithium heparin | 30 hrs | 1 mL blood or 1 x 106 recipient PBMCs 1 x 106 donor splenocytes or PBMCs (per stimulus) |
| Lymphocyte Proliferation Assay | Peripheral whole blood collected in sodium/lithium heparin | 30 hrs | 1 mL blood or 1 x 106 PBMCs |
| Natural Killer Cell Cytotoxicity Assay | Peripheral whole blood collected in sodium/lithium heparin | 30 hrs | 2 mL blood or 2 x 106 PBMCs |
| Oxidative Burst Assay | Peripheral whole blood collected in sodium/lithium heparin | 30 hrs | 200 µL whole blood |
For whole blood samples, refer to the specific assay requirements above. Samples should be collected, stored, and shipped at room temperature and will only be accepted within the timeframe listed. For isolated and cryopreserved cells, please follow the minimum cell amounts listed. From 8 mL of blood, typical PBMC yield is ~5–12 x 106. Processing and cryopreservation services are available. If isolating cells yourself, we recommend Ficoll-Paque PLUS separation. For cryopreserved samples, store in liquid nitrogen or at -80°C and ship on dry ice. For protocol questions, please contact us.
Results and Data Retrieval
All prices include FlowJo data analysis. Additional analysis is available upon request. Results include raw .fcs files, analysis reports, and gating PDFs. Turnaround is typically up to two working weeks.
Immunoassays (Luminex and ELISA)
Sample Preparation
Most assays require 15–200 µL of plasma, serum, supernatant, or lysate. Refer to your assay protocol for accepted sample types.
For plasma/serum: use appropriate collection tubes and store at -80°C.
For supernatants: store at -80°C and provide 2 mL of media used.
For lysates: normalize to 200–2,000 µg/mL protein and provide 2 mL of buffer used.
Results and Data Retrieval
Results include concentration values, standard curves, and QC data. Optional analyses (e.g., heatmaps, clustering) are available for a fee. Turnaround is typically up to two working weeks.
Real-Time Cell Lysis and Proliferation using xCELLigence
Sample Preparation
UCLA clients should prepare viable cells (>85%) and provide 50 mL of media and/or stimuli. External clients may ship frozen cells on dry ice. Include all culture conditions and special media if applicable.
Results and Data Retrieval
Normalized cell index plots over time will be provided. Turnaround time is typically up to two working weeks.