Immune Assessment Core (IAC)
About Us
The Immune Assessment Core (IAC) is a CLIA certified laboratory (#045D0642433) providing comprehensive immunological testing services for basic, clinical, and translational studies. The IAC provides both standardized and customized multi-parameter flow cytometry, multiplexed immunoassays, ultra-sensitive immunoassay technology (Simoa Quanterix), and cellular immune function assays to evaluate the innate and adaptive immune status of study subjects. The Core uses high-throughput technologies to maximize data output using small sample volumes. We work closely with our clients to assist with data analysis and interpretation to maximize knowledge gained.
Validated tests include comprehensive immunophenotyping panels, multiplexed cytokine/chemokine detection in body fluids or culture supernatants, single molecule assays (Simoa), several ELISA assays, allo- and virus-specific T cell activation, T and B cell proliferation to antigens and mitogens, NK cell cytotoxicity, and granulocyte/monocyte oxidative burst. These assays can be used to aid the diagnosis of primary immunodeficiency and for immune monitoring purposes. In addition to our various validated assays, the core performs customized assay development to meet the investigators’ needs.
The IAC is actively involved in projects aimed at standardizing immune assessment assays, including the NIH CTOT harmonization consortium and the FOCIS/NIH sponsored HIPC validation project. These consortia are focused on developing and implementing standardized protocols for testing and data analysis.
Recent Publications
Sun Y, Sen S, Parmar R, Arakawa-Hoyt J, Cappelletti M, Rossetti M, Gjertson DW, Sigdel TK, Sarwal MM, Schaenman JM, Bunnapradist S, Lanier LL, Pickering H, Reed EF. Cytotoxic KLRG1+ IL-7R- effector CD8+ T cells distinguish kidney transplant recipients controlling cytomegalovirus reactivation. Front Immunol. 2025 Feb 14;16:1542531. doi: 10.3389/fimmu.2025.1542531. PMID: 40028342; PMCID: PMC11868092
Xie Y, Irwin S, Nelson B, van Daelen M, Fontenot L, Jacobs JP, Cappelletti M, Feng H, Li Y, Koon HW. Citrulline Inhibits Clostridioides difficile Infection With Anti-inflammatory Effects. Cell Mol Gastroenterol Hepatol. 2025 Feb 7:101474. doi: 10.1016/j.jcmgh.2025.101474. Epub ahead of print. PMID: 39923847.
Yang HH, Simpson CA, Srivastava M, Bera A, Cappelletti M, Suh JD, Wang M, Beswick DM, Maxim T, Basak SK, Srivatsan ES, Fischer JL, Jacobs JP, Lee JT. Biodiversity of the Bacterial and Fungal Microbiome and Associated Inflammatory Cytokine Profile in Chronic Rhinosinusitis. Int Forum Allergy Rhinol. 2025 Jan 8. doi: 10.1002/alr.23519. Epub ahead of print. PMID: 39776217.